Improvement effects of glycyrrhizin on Helicobacter pylori-associated gastritis in rats and its mechanism / 中国药房

OBJECTIVE To investigate the improvement effects of glycyrrhizin （GL） on Helicobacter pylori （HP）-associated gastritis in rats and its mechanism. METHODS HP-associated gastritis rat model was induced by inoculating with 1×109 cfu/mL HP. The model rats were randomly divided into model group， positive control group （HP standard quadruple group）， GL low-dose， medium-dose and high-dose groups （5， 20， 50 mg/kg）， with 12 rats in each group. Another 12 healthy rats were selected as normal control group. Except the normal control group and model group were given constant volume of normal saline intragastrically， the other groups were given corresponding drugs intragastrically， once a day， for 30 consecutive days. After administration， rats received 13C urea breath test， and delta-over-baseline （DOB） was recorded； the pathological and cellular morphological changes of gastric mucosa in rats were observed， and pathological scoring was performed； the levels of interleukin-8 （IL-8）， IL-1β， tumor necrosis factor-α （TNF-α）， reactive oxygen species （ROS） and malondialdehyde （MDA） were detected in gastric mucosa of rats； mRNA expressions of high mobility group box-1 protein （HMGB1） and nuclear factor-κ-B （NF-κB）， relative expressions of nitric oxide synthases （iNOS） and HMGB1， the phosphorylation level of NF- κBp65 were also detected in rats. RESULTS Compared with normal control group， the DOB value， histopathological score of gastric mucosa， the levels of IL-8， IL-1β， TNF-α， ROS and MDA， relative expressions of HMGB1 and NF- κB mRNA， relative expressions of iNOS and HMGB1 protein and the phosphorylation level of NF-κB p65 were all increased significantly in model group （P＜0.05）； the epithelial cells of gastric mucosa in rats were incomplete in structure and decreased in the number， with an increase in cell fragments and vacuoles， and significant cell pyknosis. Compared with model group， the changes of the above indexes in GL groups and positive control group were significantly reversed （P＜0.05）； the changes in the above indicators in the GL high-dose group were more significant than GL low-dose and medium-dose groups （P＜0.05）； the pathological changes of gastric mucosal cells in rats had all improved. CONCLUSIONS GL may inhibit inflammation and oxidative stress by inhibiting the activation of HMGB1/NF-κB pathway， thus relieving HP-induced gastric mucosal injury.

Opposite effects of miR-155 in the initial and later stages of lipopolysaccharide (LPS)-induced inflammatory response / 浙江大学学报（英文版）（B辑：生物医学和生物技术）

Although microRNA-155 (miR-155) is considered a pro-inflammatory mediator, cumulative evidence indicates that it also has anti-inflammatory effects in macrophages and dendritic cells. In this study, we identified the dramatic expression changes of more than half of potential miR-155-targeted genes upon lipopolysaccharide (LPS) stimulation; 223 genes were down-regulated and 85 genes were up-regulated, including suppressor of cytokine signaling 1 (

MiR-139-5p inhibits the proliferation of gastric cancer cells by targeting Regulation of Nuclear Pre-mRNA Domain Containing 1B.

Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B) has been of great interest in the field of oncology in recent years. The relationship between miRNAs and RPRD1B in gastric cancer (GC) has not been adequately reported. This study was designed to screen RPRD1B-targeted miRNAs and investigate its regulatory mechanism in GC cells. Quantitative RT-PCR and in situ hybridization were used to detect miRNA expression in GC tissues. Colony formation, EdU cell proliferation assay, and flow cytometry were used to analyze the cell cycle. Database-assisted gene expression analysis revealed that RPRD1B was targeted and regulated by miRNA-139-5p in GC. miRNA-139-5p expression was higher in GC tissue than in normal tissues and significantly correlated with tumor size, pathological stage, and disease-free survival of GC (p < 0.05). MiRNA-139-5p regulates GC cell proliferation and affects the transition from G1 to S phase. It binds explicitly to the 2013-2019 sites of the 3'UTR of RPRD1B and negatively regulates RPRD1B expression. We demonstrated that the ability of miR-139-5p to regulate GC cell proliferation depends on RPRD1B. This process is accompanied by changes in Cyclin D1 protein expression. We established a miR-139-5p/RPRD1B/tumor proliferation axis in GC, which may serve as novel biomarkers and drug targets for GC.

Tumor deposit serves as a prognostic marker in gastric cancer: A propensity score-matched analysis comparing survival outcomes.

BACKGROUND: Gastric cancer (GC) treatment is determined by accurate tumor staging. The value of tumor deposit (TD) in prognostic prediction staging system is not yet determined. METHODS: We retrospectively analyzed clinical information on GC patients who underwent gastrectomy at the Department of General Surgery of the Chinese PLA General Hospital from July 2014 to June 2016. Propensity score matching (PSM) was performed to reduce the possibility of selection bias according to the presence of TD. RESULTS: Of the 1034 GC patients, 240 (23.21%) presented with TD, which was associated with younger age and larger tumor size (all P < .05). TD-positive patients had a worse survival than TD-negative patients before (P < .001) and after (P = .017) matching. Multivariable analysis showed that mortality risk of patients with TD increased by 58%, 62%, 37%, and 40% in the crude (HR = 1.58, 95% CI 1.32-1.89, P < .001), adjusted I (HR = 1.62, 95% CI 1.35-1.94, P < .001), adjusted II (HR = 1.37, 95% CI 1.13-1.66, P = .001), and adjusted III (HR = 1.40, 95% CI 1.16-1.68, P < .001) models before matching. Similarly, in the PSM cohort patients with TD had worse prognosis in the crude (HR = 1.32, 95% CI 1.07-1.63, P = .011), adjusted I (HR = 1.35, 95% CI 1.09-1.67, P = .005), adjusted II (HR = 1.26, 95% CI 1.00-1.58, P = .049), and adjusted III (HR = 1.33, 95% CI 1.07-1.65, P = .010) models. TD had a similar value range between N1 and N2 stages among different models. CONCLUSIONS: Among GC patients, TD is associated with survival and may have a role in the staging of patients.

Enlightenment of ambulatory patient groups on medical insurance payment system in China / 中华医院管理杂志

The current outpatient payment method based on fee-for-service induced serious demand, which increased the waste of medical insurance fund. The authors briefly introduced ambulatory patient groups(APG)with the same concept of diagnosis-related groups. According to the current situation of outpatient medical insurance payment and the degree of informatization in China, suggestions are put forward: launch a pilot project first and starting with chronic disease to promote reform; improve the quality of outpatient electronic medical records, and develop APG suitable for China; under the background of aging, cooperate with the total budget to ensure the security of medical insurance.

Application of two different nursing schemes in cancer patients with moderate and severe incontinence dermatitis / 中国实用护理杂志

Objective@#To investigate the effects of two intervention methods, hydroglue dressing and skin powder combined with skin protective film, in severe tumor patients with moderate and severe incontinence dermatitis.@*Methods@#A total of 68 patients with moderate and severe incontinence dermatitis were selected from ICU in Shanghai Oncology Hospital, and randomly divided into 2 groups by random digit table, each group included 34 cases, on the basis of the consistency of skin care, the experimental group used water adhesive dressing protection, every 1-3 days replacement, the control group used skin care powder joint skin protective film, three times a day, two groups of intervention for 10 days in a row. The healing effect, healing time, nursing time and nursing cost of IAD were observed.@*Results@#Of the 68 patients, 2 were transferred to the local hospital for treatment and 1 died. Finally, 65 patients were completed, 32 in the control group and 33 in the experimental group. In the average nursing time, the difference was statistically significant in the experimental group (1.83 ± 0.78) min and the control group (4.45 ± 0.52) min (t= 15.908, P<0.01). The experimental group took less time to care for IAD than the control group, which were (45.78 ± 25.45)min and (110.97 ± 27.22) min. The difference was statistically significant (t= 9.967, P <0.01). The cost of care in the experimental group was lower than that in the control group, which was (164.06 ± 60.32)yuan and (280 ± 0.00)yuan. The difference was statistically significant (t= 11.041, P <0.01).@*Conclusions@#The water glue dressing can be used for the nursing of moderate and severe incontinence dermatitis, and it can effectively reduce the nursing time of nursing staff and reduce the economic cost.

Application of two different nursing schemes in cancer patients with moderate and severe incontinence dermatitis / 中国实用护理杂志

Objective To investigate the effects of two intervention methods, hydroglue dressing and skin powder combined with skin protective film, in severe tumor patients with moderate and severe incontinence dermatitis. Methods A total of 68 patients with moderate and severe incontinence dermatitis were selected from ICU in Shanghai Oncology Hospital, and randomly divided into 2 groups by random digit table, each group included 34 cases, on the basis of the consistency of skin care, the experimental group used water adhesive dressing protection, every 1-3 days replacement, the control group used skin care powder joint skin protective film, three times a day, two groups of intervention for 10 days in a row. The healing effect, healing time, nursing time and nursing cost of IAD were observed. Results Of the 68 patients, 2 were transferred to the local hospital for treatment and 1 died. Finally, 65 patients were completed, 32 in the control group and 33 in the experimental group. In the average nursing time, the difference was statistically significant in the experimental group (1.83 ± 0.78) min and the control group (4.45 ± 0.52) min (t = 15.908, P<0.01). The experimental group took less time to care for IAD than the control group, which were (45.78 ± 25.45)min and (110.97 ± 27.22) min. The difference was statistically significant (t=9.967, P<0.01). The cost of care in the experimental group was lower than that in the control group, which was (164.06 ± 60.32)yuan and (280 ± 0.00)yuan. The difference was statistically significant (t=11.041, P<0.01). Conclusions The water glue dressing can be used for the nursing of moderate and severe incontinence dermatitis, and it can effectively reduce the nursing time of nursing staff and reduce the economic cost.

Application of Vitamin E in Pregnant Women with Autoimmune Thyroid Disease / 现代医院

Objective To investigate the changes of serum vitamin E in pregnant women with autoimmune thyroid diseases and provide evidence for clinical prevention of adverse pregnancy caused by oxidative stress. Methods Randomly selecting 221 cases of obstetrics and gynecology pregnant women in outpatient clinics in Chancheng District Central Hospital. A double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA) was used to detect vitamin E concentration. Results The results of 221 subjects were normally distributed. The vitamin E concentration in the disease group was (353. 93 ± 141. 28) mmol/L, and the control group was (310. 49 ± 105. 49) mmol/L. There was a significant difference between the two groups (P＜0. 05). The concentration of vitamin E in different gestational weeks gradually decreased with the increase of pregnancy, and the difference between them was statistically significant (F = 8. 417, P＜0. 05). Conclusion The presence of oxidative stress during pregnancy in women with autoimmune thyroid diseases should be valued by clinicians.

Effects of an electric standing bed and a dynamic electric standing bed on auditory evoked potential in healthy people / 中华物理医学与康复杂志

Objective To observe and compare the effect of training using an electric standing bed or a dy-namic electric standing bed on the auditory evoked potentials (BAEPs) in the brainstems of healthy people. Methods Twenty healthy people were divided into a common group ( n=10) and a dynamic group ( n=10) . The common group accepted training using an electric standing bed, while the dynamic group accepted training using a dynamic electric standing bed. Before and after the training, BAEPs were measured and compared using variance analysis. Results The latencies of the I, III and V waves among the common group were not significantly different from those of the dy-namic group before the training. After the training, however, the average wave V latency was significantly shorter than that in the common group. After the training there were, however, no significant differences in the I-III, I-V or III-V interpeak latencies in the common group, nor in the I-III interpeak latency in the dynamic group compared with before the training. In the dynamic group the average I-V and III-V interpeak latencies after the training were significantly shorter than those beforehand. However, there were no significant differences between the two groups in terms of the I-III, I-V or II-V interpeak latency after the training. Conclusion Compared with training using an electric standing bed, a dynamic electric standing bed gives significantly greater improvement in the latency and interpeak latency of BAEP waves.

Clinical profile and visual outcomes after treatment in patients with dysthyroid optic neuropathy / 基础医学与临床

Objective To review the clinical data and visual outcomes after treatment of patients with dysthyroid op-tic neuropathy ( DON) .Methods We retrospectively examined the clinic data of the 38 patients (67 eyes) suffer-ing from DON and analysed the effect of glucocorticoids , radiation and orbital decompression .Results The study included 14 men and 24 women.32 patients ( 84.2%) received multiple treatment modalities .LogMAR vision acuity changed from (0.60 ±0.59) before treatment to (0.18 ±0.09) after treatment(P<0.01) .There were 59 eyes (88.1%) in the treatment effective group , 8 eyes (11.9%) in the ineffective group .38 eyes underwent ini-tial treatment with intravenous steroid pulse therapy and 35 eyes ( 92.1%) were effective .29 eyes were treated with other modalities and 24 eyes(82.8%) were effective(P<0.01).There were 3 eyes (5.1%) suffering from fixed eyeball movement in the effective group , while 4 eyes ( 50%) in the ineffective group ( P <0.05 ) .The thickness of the supraocular muscle group in the effective group was (7.63 ±1.19 ) mm, (8.81 ±0.83 ) mm in the ineffective group (P<0.05).Mean defect of the visual field was (2.41 ±2.82) in the effective group and (11.98 ± 7.07 ) in the ineffective group ( P<0.05 ) .Conclusions Treatment with multiple modalities effectively improved visual outcomes in cases of DON .Intravenous pulse steroid therapy was recommended as the initial modality .

Effects of bioactive glass on reducing the hypersensitivity after full crown preparation / 北京大学学报(医学版)

Objective: To evaluate the efficacy of the 45S5 bioactive glass on the reduction of hypersensitivity of teeth prepared for full coverage crowns.Methods: In the study, 31 prepared abutment teeth with vital pulp from 18 patients satisfied the inclusion criteria and exclusion criteria.The patients were informed consent and recruited into the study.This was a before and after self-control, single blind clinical trial.The severity of tooth hypersensitivity at baseline was evaluated, after application of the placebo, which was the powder of zinc oxide, and after application of the 45S5 bioactive glass on the same tooth.Tooth hypersensitivity was evaluated by Yeaple probe and visual analogue scale (VAS) when the tooth was stimulated by a fixed value of mechanical pressure and a blast of cold air respectively.And the dentist gave the subjective score, and the Schiff score at the same time.The three methods of measurement were repeated at baseline, after application of placebo and after application of the powder of 45S5 bioactive glass.Student''s t test and nonparametric test were used for statistical analysis.Results: The indexes measured at baseline, after the application of the placebo and after the application of the 45S5 bioactive glass were as follows, respectively: Tactile value (29.03±9.44) g, (29.68±9.48) g and (44.19±11.19) g, VAS value (50.79±22.92) mm, (46.63±22.06) mm and (30.90±20.30) mm, Schiff score 2.13±0.67, 1.97±0.66 and 1.42±0.56.After the application of bioactive glass, the values of Tactile, the VAS, and the Schiff score were statistically different from those at baseline or after application of the placebo (P0.05).Statistical differences between the Schiff scores of the groups were associated with gender, tooth position, primary or secondary tooth preparation.Conclusion: Results of the clinical trial showed that the powders of 45S5 bioactive glass were significantly effective on reducing the hypersensitivity of teeth with vital pulp after the full crown preparation.

Comparison of simple canalith repositioning treatment and medication therapeutic alliance in the management of canalithiasis associated with benign paroxysmal positional vertigo of the horizontal semicircular canal / 临床耳鼻咽喉头颈外科杂志

Objective:To compare the therapeutic efficacy and the recurrence rate between the simple repositioning method and the reposition manoeuvre plus medication in the treatment of horizontal semicircular canal otolith benign paroxysmal positional vertigo (HSC-BPPV). Method:Sixty-two patients diagnosed with otolith HSC-BPPV by roll maneuver test were randomly divided into canalith repositioning group (32 patients) and reposition plus drug treatment group (30 patients). Patients in the canalith repositioning group were treated only with Barbecue reposition maneuver; patients in the reposition plus drug treatment group were treated firstly with Barbecue reposition maneuver and then were given Alprostadil, Cinepazide and Betahistine drug treatment. Both groups were evaluated after 7 days and 28 days treatment, and the recurrence rate was analyzed after 3 months. Result:After 7 days of the treatment , the recovery rate of the two groups was 62.5% and 73.3%, respectively. There's no significant difference between the two groups. However, the total effective power of the reposition plus drug treatment group was 96.7%, which was significantly higher than that of the canalith repositioning group (75.0%) (χ²=5.858, P<0.05). There were 8 patients in the canalith repositioning group showed changes of BBPV types after treatment, while only 1 patient in the reposition plus drug treatment group showed lesion changes. The difference was statistically significant (χ²=4.061, P<0.05). After 28 days of the treatment, the recovery rate and the total effective power of the two groups was 100%, respectively. There is no statistical difference in the total effective rate between the two groups. After 3 months follow-up, 2 patients in the canalith repositioning group (6.25%) and in the reposition plus drug treatment group (6.67%) showed BBPV recurrence, and no significant difference in the recurrence rate was found between the two groups (P>0.05). Conclusion:The repositioning maneuver is the preferred method for treating HSC BPPV. Canalith reposition maneuver plus medication has no obvious effect on the recovery rate and the recurrence rate, it only increases the effective rate and reduces the changes of the BBPV types.

Effect of different nutritional status of elderly patients with rectal cancer before surgery on postoperative reha-bilitation / 局解手术学杂志

Objective To summarize the prognosis of elderly patients with colorectal cancer excision who were divided into different groups by the nutritional status before their surgery.Methods In our hospital,100 patients (age from 64 to 70)with colorectal cancer were performed the screening of nutritional risk by NRS2002 before surgery.Patients with score from 1 to 2 were as the group A,and patients with score over 3 were as group B.The related indexes and recovery between two groups were compared.Results The NRS2002 rating scale score showed that 76 patients were at nutritional risk,37 cases among them received nutrition support and had faster recovery of intestinal function the time of tube pulled out after operation[(1.8 ±0.4)d vs.(2.9 ±1.5)d],exhaust time[(2.4 ±0.6)d vs.(4.3 ±0.9)d],time of in-take food[(2.9 ±0.5)d vs.(4.5 ±1.1)d],length of stay[(10.8 ±0.9)d vs.(14.1 ±1.3)d],incidence of infection related complica-tions (0 vs.5.13%),the differences were statistically significant(P <0.05).Conclusion Problems exist in varying degrees of loss of nu-trition in elderly colorectal cancer patients before surgery,an objective assessment of the extent and targeted care and nutritional support can significantly improve the postoperative nutrition and to facilitate recovery of gastrointestinal function after surgery.

Effects of an anti-CD86 chimeric antibody (ch1D1) on autoreactive B lymphocytes isolated from pa-tients with SLE / 中华微生物学和免疫学杂志

Objective To investigate the effects of ch1D1, an anti-CD86 chimeric antibody, on autoreactive B lymphocytes isolated from patients with systemic lupus erythematosus ( SLE) . Methods Flow cytometry analysis was performed to measure the expression of CD86 on the surface of B cells isolated from patients with SLE and to analyze the effects of ch1D1 on the activation of CD4+T cells. The method of magnetic bead sorting was used to separate B cells, NK cells and CD4+T cells from PBMC collected from healthy subjects and patients with SLE for subsequent experiments. Antibody-dependent cell-mediated cyto-toxicity (ADCC) and complement-dependent cytotoxicity (CDC) that were mediated by ch1D1 were meas-ured with LDH release assay. Effects of ch1D1 on the secretion of auto-antibodies and the proliferation of CD 4+ T were detected by ELISA and 3 H -thymidine ( 3 H-TdR) incorporation assay, respectively. Results The levels of CD80 (68. 08±14. 28 vs 46. 10±12. 14, n=24, P<0. 000 1) and CD86 (44. 72±14. 90 vs 13. 99±10. 74, n=24, P<0. 000 1) expressed on the surface of B cells isolated from patients with SLE were significantly higher than those from the healthy subjects, suggesting the abnormal activation of B cells. Com-pared with the negative control group and the murine monoclonal antibody 1D1, ch1D1 was more effective in mediating the ADCC and CDC responses (P=0. 017 2, P=0. 038 8). Activated T cells significantly en-hanced the secretion of auto-antibodies by B cells isolated from patients with SLE. Compared with the nega-tive control group, the enhanced secretion of auto-antibodies was significantly inhibited by treatment with ch1D1 (P=0. 001 9). Moreover, ch1D1 significantly inhibited the proliferation and activation of CD4+T cells induced in patients with SLE (P=0. 002 4, P=0. 049 5). Conclusion ch1D1, the anti-CD86 chim-eric antibody, could effectively mediate the ADCC and CDC responses against autoreactive B cells isolated from patients with SLE, inhibit the secretion of auto-antibodies and suppress the proliferation and activation of auto-reactive CD4+T cells. It might be a potential immunotherapy agent for the treatment of SLE.

The status, problem and progress of diabetic retinopathy treatment / 中华眼底病杂志

Laser photocoagulation,intravitreal injection of antibody against vascular endothelial growth factor (VEGF) or corticosteroids and pars plana vitrectomy are current popular therapeutic approaches for diabetic retinopathy (DR).However,some DR patients still progress to irreversible blindness even after the above treatments which do not aim at the pathological mechanisms and influence factors for DR.Thus,with the further elucidation on the molecular pathological mechanisms and overall understanding of the factors affecting DR development,more and more potential therapeutic interventions such as neuron protection,vascular reconstruction and protection,gene therapy,non-VEGF dependent antineovascularization agents have been explored.Individual precise therapy based on the potential therapeutic targets would provide the promising future for DR patients.

Accuracy of continuous noninvasive partial pressure of carbon dioxide monitoring in old diabetic patients undergoing general anesthesia / 中华麻醉学杂志

Objective To evaluate the accuracy of continuous noninvasive partial pressure of carbon dioxide monitoring in the old diabetic patients undergoing general anesthesia.Methods Sixty-six old diabetic patients of both sexes,aged 65-76 yr,weighing 49-95 kg,of American Society of Anesthesiologists physical status Ⅰ or Ⅱ,undergoing elective surgery under general anesthesia,were included in this study.Transcutaneous partial pressure of carbon dioxide (TcPCO2) was monitored by a noninvasive transcutaneous carbon dioxide monitor.Arterial blood samples were collected at 30 and 60 min after endotracheal intubation,partial pressure of arterial carbon dioxide (PaCO2) was monitored,and TcPCO2 and end-tidal pressure of carbon dioxide (PET CO2) were recorded.Bland-Altman analysis was used to measure the agreement.Results At 30 min after intubation,the results of Bland-Altman analysis showed that the mean difference between PaCO2 and TcPCO2 was 1.3,95％ confidence interval (CI) was 1.0-1.6,and the limit of agreement was-1.1-3.7;the mean difference between PaCO2 and PETCO2 was -3.2,95％CI:-3.6--2.8,and the limit of agreement was-6.6-0.2.At 60 min after intubation,the results of Bland-Altman analysis showed that the mean difference between PaCO2 and TcPCO2 was 1.4,95％ CI was 1.1-1.7,and the limit of agreement was-1.0-3.4;the mean difference between PaCO2 and PETCO2 was-3.1,95％CI was-3.5--2.7,and the limit of agreement was-6.7-0.5.The repeatability coefficients of PaCO2,TcPCO2 and PETCO2 were 2.1,2.3 and 2.3,respectively,at 30 and 60 min after intubation.Conclusion Continuous noninvasive partial pressure of carbon dioxide monitoring provides good accuracy and can be used as an alternative to PaCO2 monitoring,and the accuracy is higher than that of PETCO2 for the old diabetic patients undergoing general anesthesia.

Efficacy of transversus abdominis plane block for postoperative analgesia in pediatric patients undergoing operation on inguinal region / 中华麻醉学杂志

Objective To evaluate the efficacy of transversus abdominis plane (TAP) block for postoperative analgesia in the pediatric patients undergoing operation on the inguinal region.Methods Sixty-four pediatric patients of both sexes, aged 1-3 yr, weighing 8.5-23.6 kg, of American Society of Anesthesiologists physical status Ⅰ , undergoing elective unilateral high ligation of the hernia sac or high ligation of the processus vaginalis, were equally randomized into either intravenous analgesia group (group VA) or TAP block group (group TAP).In group TAP, after induction of anesthesia, the pediatric patients received ultrasound-guided TAP block with 0.2％ ropivacaine 1 ml/kg injected locally.After the laryngeal mask airway was inserted, the pediatric patients were mechanically ventilated, and then inhaled 2％-3％ sevoflurane for maintenance of anesthesia.In group VA, postoperative analgesia was performed with fentanyl 0.35 μg · kg-1 · h-1 until 24 h after operation.The level of pain was rated using the FLACC pain scale.When FLACC score＞5, fentanyl 0.25 μg/kg was injected intravenously as rescue analgesic.The occurrence of TAP block-related adverse events, time for removal of laryngeal mask airway, and occurrence of fentanyl-related nausea and vomiting, respiratory depression, and emergence agitation were recorded.Results No TAP block-related adverse events were observed in group TAP, and no respiratory depression was found in the two groups.Compared with group VA, the time for removal of laryngeal mask airway was significantly shortened, and the requirement for rescue analgesics and incidence of vomiting and emergence agitation were decreased in group TAP (P＜ 0.05).Conclusion TAP block with 0.2％ ropivacaine 1 ml/kg provides good efficacy for postoperative analgesia with good safety in the pediatric patients undergoing operation on the inguinal region.

Influence of carbodiimide-ethanol solution surface treatment on dentin microtensile bond strength / 北京大学学报(医学版)

Objective:To evaluate the microtensile bond strength changes and patterns of fractures of the bonding interface after dentine surface treatment with carbodiimide-ethanol solution .Methods:1-eth-yl-3-(3-dimethylaminopropyl) carbodiimide (EDC) dissolved in ethanol was diluted into different con-centrations of 2, 1, 0.3, 0.1 and 0.01 mol/L EDC-ethanol solutions.Twenty-eight caries-free extracted human third molars were ground metallurgically to prepare flat occlusal mid-coronal dentin surfaces and etched with 35%( mass fraction) phosphoric acid gel .Then they were treated with EDC-ethanol solution for 60 s before the bonding procedure and randomly divided into five experimental groups corresponding to the tested EDC-ethanol concentrations .The ethanol treated and no pre-treated surfaces were used as con-trols.Single Bond 2 adhesive was applied and resin composite disk was stacked on the treated dentine surface .The teeth with resin composite disks were stored in water at room temperature for 24 h and then sectioned longitudinally to produce stick specimens for microtensile bond strength test .Fracture patterns were observed with a stereomicroscope .Results:The dentin surfaces pre-treated with 2 mol/L [ (22.17 ± 13.31) MPa] and 1 mol/L [(45.31 ±17.80) MPa] EDC-ethanol solutions resulted in statistically sig-nificant lower bond strength value ( P0.05).Conclusion:No significant difference of immediate bond strengths was found in the 0.3, 0.1, 0 .01 mol/L groups compared with the control group .EDC-ethanol solution surface treatment with con-centrations of 2 mol/L and 1 mol/L resulted in decreasing of the bonding strength .

Study on the immunosuppressive activities of myeloid derived suppressor cells in patients suffering from recurrent spontaneous abortion / 中华微生物学和免疫学杂志

normal pregnancy and RSA (P=0.0250).Compared with women with normal pregnancy, those with RSA showed significantly decreased levels of Arg-1 ( P=0.0474) and suppressed activities of MDSCs against CD8+T cell proliferation (P=0.0230).Conclusion The MDSCs might play an important role in the main-tenance of maternal immune tolerance.Decreased number and inhibited function of MDSCs in the peripheral blood of women with RSA might be responsible for the development of RSA.

Effect of comprehensive schistosomiasis control measures based on infection source control in mountainous areas of Yunnan Province / 中国血吸虫病防治杂志

Objective To evaluate the effect of comprehensive schistosomiasis control measures based on infection source control in plateau mountain areas of Yunnan Province. Methods From 2006 to 2004,four administrative villages were selected as test areas from plateau canyon and plateau basin endemic areas in Jindun Town,Heqing County,two villages each type,and the comprehensive control measures were implemented,including the examination and treatment of schistosomiasis,Oncomela?nia hupensis snail survey and control,health education,improving drinking water and lavatories,banning grazing,constructing sanitary pen of livestock,replacing cattle with machine,etc. The schistosome infection state and snail status in 2006 were treat?ed as the baseline information,and the effect of the comprehensive measures were evaluated. Results The infection rate of hu?man in plateau canyon areas decreased from 4.94%in 2006 to 0.06%in 2014,and that of livestock decreased from 1.11%to 0. In plateau basin areas,there was only 1 case of schistosomiasis found in Xiaolian Village in 2007,and no any other cases found in the other years,the infection rates of livestock dropped from 7.38%to 0. Compared with 2006,the snail areas in the two type areas decreased by 74.89%and 75.30%,respectively,meanwhile,the percentage of snail area,the occurrence rate of frames with snails,as well as the average density of living snails also decreased,and no infected snails were found since 2008. Xidian and Xinzhuang villages in plateau canyon area reached the criteria of schistosomiasis transmission controlled in 2009,and Xiao?lian and Kangfu villages in plateau basin reached the criteria of transmission interrupted in 2014. Conclusions The comprehen?sive schistosomiasis control measures based on infection source control can effectively control the endemic situation of schistoso? miasis in plateau areas of Yunnan Province. In the future,we should pay an equal attention to the infection sources control and snail control to consolidate and amplify the achievement of schistosomiasis control.